Coding
osmY

Part:BBa_K892008:Experience

Designed by: David Zong, Felix Ekness   Group: iGEM12_Washington   (2012-09-30)


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Applications of BBa_K892008

User Reviews

UNIQda16058bcc138679-partinfo-00000000-QINU

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iGEM Dundee 2016

This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter BBa_K914003, a strong RBS BBa_B0030) and OsmY amplified with a C-terminal HA tag resulting in the following composite part BBa_K1963007. The device showed secretion of OsmY under rhamnose-regulated conditions.

We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli BBa_K1963005 and Serratia BBa_K1963006). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the rhamnose promoter in order test for rhamnose-regulated expression of an sRNA secretion system



User Reviews

UNIQda16058bcc138679-partinfo-00000007-QINU UNIQda16058bcc138679-partinfo-00000008-QINU