Part:BBa_K892008:Experience

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User Reviews

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••••• iGEM Dundee 2016

This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter BBa_K914003, a strong RBS BBa_B0030) and OsmY amplified with a C-terminal HA tag resulting in the following composite part BBa_K1963007. The device showed secretion of OsmY under rhamnose-regulated conditions. We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli BBa_K1963005 and Serratia BBa_K1963006). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the rhamnose promoter in order test for rhamnose-regulated expression of an sRNA secretion system

User Reviews

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